Analysis of Nucleic Acids by Capillary Electrophoresis (eBook, PDF)

Redaktion: Heller, Christoph

• Format: PDF

Produktbeschreibung

Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.

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Produktdetails

• Verlag: Vieweg+Teubner Verlag
• Seitenzahl: 314
• Erscheinungstermin: 29. Juni 2013
• Englisch
• ISBN-13: 9783322910158
• Artikelnr.: 53141144

Autorenporträt

Dr. Christoph Heller ist Wissenschaftler am Max-Planck-Institut für molekulare Genetik in Berlin. The editor, Dr. Christoph Heller, is currently working at the Max-Planck-Institute for Molecular Genetics in Berlin-Dahlem, Germany. Since 1985 he is working in the field of DNA separation, both in conventional gels and - since five years - also in capillaries.

Inhaltsangabe

I Basic Concepts.- 1 The Separation Matrix.- 2 Electrophoresis Theories.- 3 Microscopic Studies on the Migration Mechanism.- II Factors Affecting the Separation.- 4 Influence of Polymer Concentration and Electric Field.- 5 Effects of Sample Matrix and Injection.- 6 Developments in Capillary Coating and DNA Separation Matrices.- III Applications.- 7 Analysis of Oligonucleotides and Antisense DNA Analogs.- 8 Separation of DNA Restriction Fragments and PCR Products.- 9 Pulsed-Field Capillary Electrophoresis of Large DNA.- 10 Capillary Electrophoresis for DNA Sequencing.- 11 Mutational Analysis with Capillary Electrophoresis.- 12 Analysis of Transfer RNA and 5S Ribosomal RNA.- IV Recent Developments.- 13 Update on Improvements in DNA Separation.