Z-DNA

Methods and Protocols
Herausgegeben:Kim, Kyeong Kyu; Subramani, Vinod Kumar

• Gebundenes Buch

Produktbeschreibung

This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z.
Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This is an open access book.

Produktdetails

• Methods in Molecular Biology 2651
• Verlag: Humana / Max-Planck-Institut für Multidisziplinäre Naturwissenschaften / Springer US / Springer, Ber
• Artikelnr. des Verlages: 978-1-0716-3083-9
• 2023
• Seitenzahl: 348
• Erscheinungstermin: 10. März 2023
• Englisch
• Abmessung: 260mm x 183mm x 25mm
• Gewicht: 852g
• ISBN-13: 9781071630839
• ISBN-10: 1071630830
• Artikelnr.: 66887082

Inhaltsangabe

The Origin of Left-Handed Poly[d(G-C)].- Characterization of Z-DNA Using Circular Dichroism.- Characterization of Z-DNA by Infrared Spectroscopy.- Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein.- NMR Titration Studies in Z-DNA Dynamics.- Single-Molecule Methods to Study Z-DNA Mechanics and Dynamics.- BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector.- Oligonucleotide Containing 8-Trifluoromethyl-2'-Deoxy-Guanosine as a Z-DNA Probe.- Chiroptical Properties of Z-DNA Using Ionic Porphyrins and Metalloporphyrins.- Construction of a Z-DNA Specific Recombinant Nuclease Z -FOK for Conformation Studies.- Human Heme Oxygenase-1 Promoter Activity is Mediated by Z-DNA Formation.- ChIP-Seq Strategy to Identify Z-DNA Forming Sequences in the Human Genome.- Detection of Z-DNA Structures in Supercoiled Genome.- Thermogenomic Analysis of Left-Handed Z-DNA Propensities in Genomes.- DeepZ: A Deep-Learning Approach for Z-DNA Prediction.- Methods to Study Z-DNA-Induced Genetic Instability.- Single-Molecule Visualization of B-Z Transition in DNA Origami Using High-Speed AFM.- Adoption of A-Z Junctions in RNAs by Binding of Z Domains.- Detecting Z-RNA in Mammalian Cells.- Identification of ADAR1 p150 and p110 Associated Edit Sites.- Z-DNA and Z-RNA: Methods Past and Future.