X-Chromosome Inactivation

Methods and Protocols
Herausgegeben:Sado, Takashi

• Gebundenes Buch

Produktbeschreibung

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.

Produktdetails

• Methods in Molecular Biology 1861
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8765-8
• 1st ed. 2018
• Seitenzahl: 264
• Erscheinungstermin: 15. September 2018
• Englisch
• Abmessung: 260mm x 183mm x 20mm
• Gewicht: 629g
• ISBN-13: 9781493987658
• ISBN-10: 1493987658
• Artikelnr.: 52788148

Inhaltsangabe

Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs.- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library.- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library.- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos.- Somatic Cell Nuclear Transfer in Mice - Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status.- Live Imaging of Xist RNA.- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics.- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes.- Visualization of Hidden Epitopes at the Inactive X Chromosome.- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation.- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos.- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos.- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos.- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation.- X-chromosome Inactivation and Escape from X inactivation in Mouse.- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles.