In Uses of Immobilized Biological Compounds the reader will find a comprehensive survey of the field written by acknowledged experts who met in Brixen, Italy, between May 9 and 14, 1993 for a NATO Advanced Research Workshop devoted to the topic. The resulting volume presents a critical review of the latest results in the area and sets guidelines for future research. The 53 reports presented here cover: (A) General Aspects of Immobilizing Biological Compounds; (B) Medical, Clinical and Pharmaceutical Applications; (C) Electrochemical Biosensors; (E) Defense Applications; (F) Immunosensors and…mehr
In Uses of Immobilized Biological Compounds the reader will find a comprehensive survey of the field written by acknowledged experts who met in Brixen, Italy, between May 9 and 14, 1993 for a NATO Advanced Research Workshop devoted to the topic. The resulting volume presents a critical review of the latest results in the area and sets guidelines for future research. The 53 reports presented here cover: (A) General Aspects of Immobilizing Biological Compounds; (B) Medical, Clinical and Pharmaceutical Applications; (C) Electrochemical Biosensors; (E) Defense Applications; (F) Immunosensors and Receptors; (G) Food, Environmental, Clinical and Analytical Applications; and (H) Biotechnology and Marketing. In short, all aspects of the area are presented, in a compact format which will appeal to undergraduates, technicians, and professional scientists in the food, clinical, environmental, pharmaceutical and industrial fields.
Immobilization of Biological Species on Bilayer Lipid Membranes.- Polymeric Environments for Proteins and Whole Cells in Molecular Sensors.- Use of Red Blood Cells (RBC) as Carriers of Bioactive Compounds.- Theoretical and Experimental Aspects for Improvement of Electrochemical Biosensors by Various Kinds of Immobilization.- Development and Characterization of Polymeric Biomaterials for Bioactive Agent Immobilization.- Two-Dimensional (Glyco)protein Crystals as an Immobilization Matrix for Functional Molecules.- Application of Graphite-Epoxy Composites in the Construction of Electrochemical Sensors and Biosensors.- Novel PHEMA Membranes for Potential Bioelectrode Use.- Immobilization of Cell Cycle Specific Drug DFMO for Chemotherapy.- Use of Immobilized Biological Compounds in Drug Analysis.- In-Vivo Monitoring with Microdialysis Probe.- Immobilised Catalase and Hydrogen Peroxide. A Novel Contact Lens Disinfection System.- Ex-Vivo Sensors; Microencapsulation of Enzyme Layer in Biosensors.- Clinical Application of Biosensing with Amperometric Detection of Ammonia-Nitrogen.- Therapeutic Potential of Immobilized Enzymes.- Optimization of Membranes with Glucose Oxidase for Glucose Determination Using Oxygen Electrode.- Multienzyme Biosensors. Coupled Enzyme Reactions and Enzyme Activation.- Three Different Approaches for Preparing GLOD and GLDH Reactors. Application for the Assay of Glutamates in Real Samples Using a FIA System.- Enzyme Immobilization on Chitin and Chitosan for Construction of Enzymatic Sensors.- Electrochemical Biosensors.- Novel Approaches to the Development of Mediated Biosensors and Enzyme Assay.- Poly(Amphiphilic Pyrrole)-Enzyme Electrode: A New Approach for Biosensor Construction.- Effect of Organic Solvents on the Behaviour of a Glucose Oxidase-BasedBiosensor.- Organic-Phase Biosensors.- Modified Enzyme Electrodes.- Can New Biosensors be Deduced from Sensing in Biology?.- Microbial Sensors and Microbiosensors.- Stimulus-Response Measurements on Protein Containing Membranes Deposited on an ISFET Surface.- Micro Sensors in Bioelectroanalysis.- Surface Characteristics of Chemical Sensors.- Optrodes for Measuring Enzyme Activity and Inhibition.- Luminescence Detection of Biological Reactions.- Progress in Fiber-Optic Based Biosensors at the United States Naval Research Laboratory.- Immobilized Biological Compounds in Defense Applications.- Biosensors in the Detection of Biological Agents.- Biosensors for Toxins and Bacteria.- Reusable Fiber Optic Biosensors for Detection of Drugs and Toxicants.- The Biosensor Technology Program at NIST.- FIA Immunoassays with Electrochemical and Optical Detection.- Immunoassay Devices for Extra-Laboratory Measurements of Toxic Chemicals Based on Capillary Migration and Liposome Amplification.- Direct Immunoassays: Limitations on the Physical Principles and on the Technology State of the Art.- Comparison of Different Solid Phases for an ELISA Based Determination of Aflatoxin B.- Polystyrene and Poly(styrene/acrolein) Latexes with Immobilized Proteins as a Basis of the Diagnostic Agglutination Test.- An Integrated Optical Immunosensor.- Immobilized Biological Compounds in Food Analysis.- Immobilized Biomolecules for Detection of Environmental Pollutants.- Enzyme Inhibition Sensor for Organophosphorus Pesticides Analysis.- Biosensors for Electrochemical Non Invasive Analysis.- Flow Through Biosensors in Clinical Analysis.- Flow Injection Analysis: A Complementary or Alternative Concept to Biosensors.- Heat Sensitive Biosensors.- The Market for Biosensors. The Strengths and the Competition.- Report on Biosensor Technology in Europe.- Author Index.
Immobilization of Biological Species on Bilayer Lipid Membranes.- Polymeric Environments for Proteins and Whole Cells in Molecular Sensors.- Use of Red Blood Cells (RBC) as Carriers of Bioactive Compounds.- Theoretical and Experimental Aspects for Improvement of Electrochemical Biosensors by Various Kinds of Immobilization.- Development and Characterization of Polymeric Biomaterials for Bioactive Agent Immobilization.- Two-Dimensional (Glyco)protein Crystals as an Immobilization Matrix for Functional Molecules.- Application of Graphite-Epoxy Composites in the Construction of Electrochemical Sensors and Biosensors.- Novel PHEMA Membranes for Potential Bioelectrode Use.- Immobilization of Cell Cycle Specific Drug DFMO for Chemotherapy.- Use of Immobilized Biological Compounds in Drug Analysis.- In-Vivo Monitoring with Microdialysis Probe.- Immobilised Catalase and Hydrogen Peroxide. A Novel Contact Lens Disinfection System.- Ex-Vivo Sensors; Microencapsulation of Enzyme Layer in Biosensors.- Clinical Application of Biosensing with Amperometric Detection of Ammonia-Nitrogen.- Therapeutic Potential of Immobilized Enzymes.- Optimization of Membranes with Glucose Oxidase for Glucose Determination Using Oxygen Electrode.- Multienzyme Biosensors. Coupled Enzyme Reactions and Enzyme Activation.- Three Different Approaches for Preparing GLOD and GLDH Reactors. Application for the Assay of Glutamates in Real Samples Using a FIA System.- Enzyme Immobilization on Chitin and Chitosan for Construction of Enzymatic Sensors.- Electrochemical Biosensors.- Novel Approaches to the Development of Mediated Biosensors and Enzyme Assay.- Poly(Amphiphilic Pyrrole)-Enzyme Electrode: A New Approach for Biosensor Construction.- Effect of Organic Solvents on the Behaviour of a Glucose Oxidase-BasedBiosensor.- Organic-Phase Biosensors.- Modified Enzyme Electrodes.- Can New Biosensors be Deduced from Sensing in Biology?.- Microbial Sensors and Microbiosensors.- Stimulus-Response Measurements on Protein Containing Membranes Deposited on an ISFET Surface.- Micro Sensors in Bioelectroanalysis.- Surface Characteristics of Chemical Sensors.- Optrodes for Measuring Enzyme Activity and Inhibition.- Luminescence Detection of Biological Reactions.- Progress in Fiber-Optic Based Biosensors at the United States Naval Research Laboratory.- Immobilized Biological Compounds in Defense Applications.- Biosensors in the Detection of Biological Agents.- Biosensors for Toxins and Bacteria.- Reusable Fiber Optic Biosensors for Detection of Drugs and Toxicants.- The Biosensor Technology Program at NIST.- FIA Immunoassays with Electrochemical and Optical Detection.- Immunoassay Devices for Extra-Laboratory Measurements of Toxic Chemicals Based on Capillary Migration and Liposome Amplification.- Direct Immunoassays: Limitations on the Physical Principles and on the Technology State of the Art.- Comparison of Different Solid Phases for an ELISA Based Determination of Aflatoxin B.- Polystyrene and Poly(styrene/acrolein) Latexes with Immobilized Proteins as a Basis of the Diagnostic Agglutination Test.- An Integrated Optical Immunosensor.- Immobilized Biological Compounds in Food Analysis.- Immobilized Biomolecules for Detection of Environmental Pollutants.- Enzyme Inhibition Sensor for Organophosphorus Pesticides Analysis.- Biosensors for Electrochemical Non Invasive Analysis.- Flow Through Biosensors in Clinical Analysis.- Flow Injection Analysis: A Complementary or Alternative Concept to Biosensors.- Heat Sensitive Biosensors.- The Market for Biosensors. The Strengths and the Competition.- Report on Biosensor Technology in Europe.- Author Index.
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