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This issue of Thoracic Surgery Clinics, guest edited by Dr. Gaetano Rocco, is devoted to Single-port/Uniportal Video-Assisted Thoracic Surgery. Dr. Rocco has assembled expert authors to review the following topics: The Geometric and Ergonomic Appeal of Uniportal VATS; Management of Intraoperative Difficulties During Uniportal VATS; Hybrid Theatre and Uniportal VATS: the Perfect Match for Lung Nodule Localization; Important Technical Details in Uniportal VATS Standard Major Pulmonary Resections; When Uniportal VATS is Contraindicated; Subxyphoid Uniportal VATS Procedures; Uniportal VATS Segmentectomy; The Case for Non Intubated/Awake Anesthesia for Uniportal VATS Procedures; Uniportal VATS Esophagectomy; Complex Pulmonary Resections Through Uniportal VATS; Training in Uniportal VATS; Fast-tracking Patients Through the Diagnostic and Therapeutic Pathways of Intrathoracic Conditions: the Role of Uniportal VATS; and more!