Type-1 Diabetes

Methods and Protocols
Herausgeber: Wang, Ping; Moore, Anna

• Gebundenes Buch

Produktbeschreibung

This volumes details methods focusing on technological innovation and recent advances in diabetes management. Chapters will guide readers through recent advances, beta-cell regeneration, non-invasive imaging of endogenous, transplanted islets theranostics, microRNA profiling of beta-cells and artificial intelligence, and deep learning algorithms in diabetes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Type-1 Diabetes: Methods and Protocols is for a broad audience including basic researches, clinicians, and physician scientists whose major focus is in diabetes.

Produktdetails

• Methods in Molecular Biology Nr.2592
• Verlag: Springer US / Springer US, New York, N.Y.
• 1st ed. 2023
• Seitenzahl: 264
• Erscheinungstermin: 13. Dezember 2022
• Englisch
• Abmessung: 260mm x 183mm x 20mm
• Gewicht: 700g
• ISBN-13: 9781071628065
• ISBN-10: 1071628062
• Artikelnr.: 64383455

Inhaltsangabe

3D optical molecular imaging of the rodent pancreas by OPT and LSFM.
 Uncommon Transplantation Sites
Transplantation of islets and islet organoids in the anterior chamber of the eye of rodents and monkeys.
 Generation of Insulin
producing multicellular organoids.
 Non
invasive quantitative PET Imaging in humans of the Pancreatic Beta Cell Mass biomarkers VMAT2 and Dopamine, D2/D3 receptors, in vivo.
 In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation.
 Diabetes retinopathy: new ways to detect and treat.
 MRI methods for imaging beta
cell function in the rodent pancreas.
 MicroRNAs in type 2 diabetes
focus on microRNA profiling in Islets of Langerhans.
 Protocol for clinical GLP
1 receptor PET/CT imaging with [68 Ga]Ga
NODAGA
exendin
4.
 Hyperinsulinemic
euglycemic clamp in conscious rats based on the tail artery and vein catheterization.
 Synthesis of siRNA
conjugated dextran
coated iron oxide nanoparticles for islet protection during transplantation and noninvasive imaging.
 Micelle
Based Nanocarriers for Targeted Delivery of Cargo to Pancreas.
 Magnetic particle imaging of transplanted human islets using a machine learning algorithm.
  In vivo bioluminescence for the detection of the fate of pancreatic islet organoids post
transplantation.
 miRNA theranostic nanoparticles promote pancreatic beta cell proliferation in type 1 diabetes model.
 Isolation and purification of human pancreatic islets.
 Vascularized islet transplantation as composite Islet
Kidney grafts with nanoparticle
labeled islets in large animal preclinical transplant models.