Michelle Arya

The Contribution of Pigmentation towards UVB Radiation Protection

In A Mouse Model of Human Melanoma

• Broschiertes Buch

Produktbeschreibung

Ultraviolet B (UVB) radiation is the component of sunlight that damages DNA. Red-haired people are especially vulnerable to the damaging effects of UVB due to the low content of eumelanin in their skin. Accumulated damage from UVB can lead to skin cancer. A transgenic strain of mouse containing an inducible melanogenesis system was used to investigate the mechanism of protection of eumelanin. Melanogenesis in this murine model can be induced by the application of the diterpene, forskolin to the epidermis. Forskolin increases eumelanin protection by inducing the expression of cAMP, which then upregulates the expression of melanogenic genes. To assess the protection afforded by eumelanin to the epidermis, transgenic mice were exposed to varying doses of UVB in the presence or absence of forskolin induced melanin. DNA damage was assessed by immunohistochemical staining for cyclobutane pyrimidine dimers. Vehicle treated mice and albino mice showed thymine dimer formation at 20 and 50mJ/cm^2 of UVB. Forskolin treated and black-skinned mice showed no thymine dimer formation at these doses. These results indicate direct UVB protection by eumelanin through a light filtering process.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 56
• Englisch
• ISBN-13: 9783845417004
• ISBN-10: 3845417005
• Artikelnr.: 33775190

Autorenporträt

The author is a PhD candidate at Tufts University Sackler School of Graduate Biomedical Sciences, Genetics. She completed her ALM from Harvard University in 2006 and received her BSc from Concordia University, Montreal, Canada in 1995. Prior to embarking on graduate studies she worked as a researcher at Eisai Research Institute in Andover, MA.