Neha Verma, Vikrant Abbot, Mrinal Sharma

Systematic Analysis of Working Models to Detect Thioredoxin Activities

Detecting Thioredoxin Activity by Systemic Analytical Tools

• Broschiertes Buch

Produktbeschreibung

Thioredoxin (Trx) (12 kDa M.W.) is a ubiquitous antioxidant protein which controls the oxidative stress and redox signaling with thioredoxin reductase (TrxR) and NADPH. Trx acts as an electron donor for ribonucleotide reductase (RNR), methionine sulfoxide reductase (Msr), and Trx peroxidase (peroxiredoxin). Trx plays an important role in DNA synthesis, reduction of the disulfide bond, recycling of peroxiredoxin (Prx), and as an electron donor. Various in vivo and in vitro assays are used to check the activity of Trx. This work is focused on the study of in vivo and in vitro methods for Trx activity which have been used in the different model system. Different methods were studied to evaluate the activity of Trx in different samples. Insulin reduction assay is more commonly used in vitro method because insulin acts as effective substrate for activity of Trx. Fluorescence, DTNB (5, 5 -dithiobis (2-nitrobenzoic acid)) reduction, spectrophotometric or micro-method insulin reduction, and disulfide cross-linking interaction in vitro assays while Luciferase, cloning, and wort in vivo assays are used for the activity of the Trx system.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 52
• Erscheinungstermin: 6. November 2023
• Englisch
• Abmessung: 220mm x 150mm x 4mm
• Gewicht: 96g
• ISBN-13: 9786206844068
• ISBN-10: 6206844064
• Artikelnr.: 69553846

Autorenporträt

Ms. Neha Verma worked as Assistant Professor at PCTE Group of Institutes, Ludhiana, Punjab.Dr. Vikrant Abbot works as Associate Professor and Head of Department at Department of Pharmacy, Chandigarh Group of Colleges, Jhanjeri, Mohali, Punjab.Ms. Mrinal Sharma works as Assistant Professor at Saraswati College of Pharmacy, Gharuan, Mohali, Punjab.