Synthesis of natural and isotope-labeled tRNA nucleosides and their mass spectrometric quantification
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In cellular nucleic acids there are more than 100 modified nucleosides, which fine-tune and regulate the activity of most RNAs and of genes in DNA. Due to these important assignments these modifications have stimulated research for decades and thousands of publications describe their occurrences, biosyntheses, and functions. Most knowledge has been obtained by the study of individual modifications, although it is known that they generally occur concertedly and bias each other.
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