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Book comprises a one pot synthesis (modified Kröhnke method) of terpyridines, their characterization. Synthesized terpyridines and 2,9-dimethyl-1,10-phenanthroline were employed to synthesize octahedral perchlorates of Ru(II). complexes are evaluated for their antimicrobial activity using Zone of inhibition and MIC, Nuclease activity was studied by using Viscosity measurement supported by Absorption Titration.