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In this chapter the new synthesized series of sulfonamide (sulfathiazol STH) containing 2-azetidinone were established by physical properties like melding point (M.P) and characterized done by elemental analysis (CHNS) also various detection spectral techniques such as FT-IR, 1H NMR, 13C NMR, and ESI-MS (Mass spectroscopic). The purities of synthesized final scaffolds have been tested by thin layer chromatography (TLC) the bioactivity of all compounds were evaluated by such as anti-bacterial activity against gram +ve and gram -ve strains and antifungal activity. The all results for scaffolds compare against standard drug. Also studied their MIC (minimal inhibitory concentration)