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This present book focuses on Synthesis and Characterization study of Isatin Hydrazone scaffolds. The major method and modification are analyzed. The book also entitle easiest routes for synthesis of Isatin Hydrazone scaffolds. We tried to include 1H NMR,13C NMR data and mass spectroscopic data for analysis of structure. It is also overviews the current green chemistry approach and Biological Importance of Isatin derivatives.