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The central role of RNA in many cellular processes has created a high interest in the specific tasks RNA molecules and protein: RNA complexes perform in a cell. In this publication up-to-date experimental methods and protein:RNA interaction protocols were used to identify the native RNA binding motive and to characterize the key role of the protein ribosomal binding factor A(RbfA). To gain insights into the binding mode of RbfA toits target RNA, two RbfA constructs from Thermotoga maritima and Helicobacter pylori were employed. Comparison of the RbfA proteins with the published homologous…mehr

Produktbeschreibung
The central role of RNA in many cellular processes has created a high interest in the specific tasks RNA molecules and protein: RNA complexes perform in a cell. In this publication up-to-date experimental methods and protein:RNA interaction protocols were used to identify the native RNA binding motive and to characterize the key role of the protein ribosomal binding factor A(RbfA). To gain insights into the binding mode of RbfA toits target RNA, two RbfA constructs from Thermotoga maritima and Helicobacter pylori were employed. Comparison of the RbfA proteins with the published homologous structure from Escherichia coli, led to studies concerning the structural attributes between proteins from thermophilic and mesophilic systems. The different chapters detail the biophysical and biochemical methods used to investigatethe protein and protein: RNA structures and their function and the advances in understanding the biological processes in which the RbfA protein is involved in.
Autorenporträt
Steffen Grimm wurde 1970 im thüringischen Sondershausen geboren. Nach Abschluss der polytechnischen Oberschule und einer anschließenden Berufsausbildung zum Elektromonteur in der DDR blieb er nach der politischen Wende 1989/90 in den alten Bundesländern und arbeitete hier in der freien Wirtschaft. Nachdem er den Wehrdienst absolviert hatte, ging er 1999, zunächst im mittleren Dienst, zur Thüringer Polizei. 2007 erhielt er hier die Möglichkeit zu einem Studium zum gehobenen Polizeidienst, in dessen Zusammenhang auch die vorliegende Studie entstand. Eigenes Interesse und bis dahin unzureichende Aufarbeitung des Themas, motivierten zur Studie und einer eindringlichen Recherche in den Archiven in Berlin, Weimar und Buchenwald. Der Autor lebt und arbeitet nach wie vor in Sondershausen/Thüringe