Somatic Embryogenesis in Strawberry
Induction , control and application of somatic embryogenesis in strawberry
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This study was conducted to examine the possibility of inducing somatic embryogenesis in strawberry using tissue culture technique. The process of somatic embryogenesis was further studied utilizing different combinations of plant growth regulators, explants sources and cultivars. Structures resembling somatic embryos were formed at high frequencies from petiole segments on medium amended with 4.0mg/l 2,4-D+ 0.5mg/l BA + 50 g/l sucrose (Exp1), or medium with 1.5 mg/l TDZ +4.0 mg/l Picloram (Exp. 3), while medium with several combinations OF BA + glutamin only formed callus (Exp.2). However, hi...
This study was conducted to examine the possibility of inducing somatic embryogenesis in strawberry using tissue culture technique. The process of somatic embryogenesis was further studied utilizing different combinations of plant growth regulators, explants sources and cultivars. Structures resembling somatic embryos were formed at high frequencies from petiole segments on medium amended with 4.0mg/l 2,4-D+ 0.5mg/l BA + 50 g/l sucrose (Exp1), or medium with 1.5 mg/l TDZ +4.0 mg/l Picloram (Exp. 3), while medium with several combinations OF BA + glutamin only formed callus (Exp.2). However, histological studies on the regenerated structures did not confirm their embryogenic nature, indicating that strawberry could be recalcitrant in term of somatic embryogenesis.
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