Single Cell ¿Omics of Neuronal Cells

Herausgegeben:Sweedler, Jonathan V.; Eberwine, James; Fraser, Scott E.

• Broschiertes Buch

Produktbeschreibung

This volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory.

Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Produktdetails

• Neuromethods 184
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-0716-2527-9
• 1st ed. 2022
• Seitenzahl: 308
• Erscheinungstermin: 12. August 2023
• Englisch
• Abmessung: 254mm x 178mm x 17mm
• Gewicht: 583g
• ISBN-13: 9781071625279
• ISBN-10: 1071625276
• Artikelnr.: 68437141

Inhaltsangabe

Singe Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping.- Single Cell Genomic and Epigenomic DNA Analyses.- Single-Cell Proteome Profiling of Neuronal Cells.- Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVI.- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology.- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells.- Single-Cell Resolution Immunoblotting.- Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis Platform.- Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative Techniques.- Electrophysiology and Single Cells.- Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells.