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The anisotropic silver nano-particles were synthesized by using a simple, efficient and eco-friendly method in the presence of Taxus baccata extract as reducing and stabilizing agent. The colloidal monodispersed nanoparticles were characterized by different methods including UV-Vis spectroscopy, SEM, TEM, AFM, EDS, FTIR, TGA, DLS and Zetasizer. Following the investigation of anticancer activity of the nanoparticles on MCF-7, Caov-4 and HeLa cancer cell lines using MTT assay, its mechanism was further investigated using flow cytometry and real-time PCR.