Quantitative Proteomics by Mass Spectrometry

Herausgegeben:Sechi, Salvatore

• Broschiertes Buch

Produktbeschreibung

Quantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology(TM) series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers.

Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS..

Produktdetails

• Methods in Molecular Biology 359
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-648-1
• Softcover reprint of hardcover 1st ed. 2007
• Seitenzahl: 228
• Erscheinungstermin: 9. Dezember 2010
• Englisch
• Abmessung: 229mm x 152mm x 13mm
• Gewicht: 337g
• ISBN-13: 9781617376481
• ISBN-10: 1617376485
• Artikelnr.: 32209130

Inhaltsangabe

Acrylamide-A Cysteine Alkylating Reagent for Quantitative Proteomics.- Using Stable Isotope Tagging and Mass Spectrometry to Characterize Protein Complexes and to Detect Changes in Their Composition.- Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics.- Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope Labeling.- The Absolute Quantification Strategy.- Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling.- The Use of a Quantitative Cysteinyl-Peptide Enrichment Technology for High-Throughput Quantitative Proteomics.- An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling.- Proteolytic Labeling With 18O for Comparative Proteomics Studies.- Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening.- Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags.- Computational Analysis of Quantitative Proteomics Data Using Stable Isotope Labeling.- Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues.- Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks.

Rezensionen

From the reviews:
"Quantitative Proteomics by Mass Spectrometry describes protocols of various quantitative proteomics procedures utilizing mass spectrometry. ... The publication of this book is timely. ... provides useful information regarding sample preparation/handling, reagent preparation/handling, mass spectrometry analysis etc. for successful experiments. ... In summary, the book serves as an excellent guide for both the experienced investigator and novice in the field who is contemplating using quantitative proteomics by mass spectrometry." (Masaru Miyagi, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)