Quantitative detection of equine babesiosis by molecular techniques
Raquel Martins Porto
Broschiertes Buch

Quantitative detection of equine babesiosis by molecular techniques

Quantification of equine babesiosis by real-time PCR - an alternative to the ELISA test

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Equine babesiosis is one of the main parasitic diseases of horses and causes great damage to animal health, so detecting it as soon as possible makes treatment more successful. The aim of this book was to apply the real-time PCR technique (Q-PCR) to quantify equine Babesiosis using primers specific to the Babesia equi haemoparasite. The method used was the extraction of blood DNA from horses followed by amplification with specific primers for Babesia equi parasites. Two types of test were carried out: nested-PCR and Q-PCR to identify and quantify the parasite. Seven horses of the breed were an...