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This book delves into the fascinating world of enzymes and their potential applications in various industries. The book focuses on amylase, an enzyme that plays a critical role in carbohydrate metabolism and is used in various industrial processes such as food, pharmaceuticals and biofuels. This book the isolation, optimization, and purification of amylase from bacteria that are naturally found in ascidians, a group of marine invertebrates. It details the methods used for isolating and optimizing the production of amylase, as well as the purification process required to obtain a high-quality enzyme. The book's comprehensive analysis of amylase's properties, including its stability and activity under various conditions, provides a deeper understanding of its potential applications in different industries. The author's research also highlights the importance of exploring natural sources of enzymes and their potential in various industrial processes. This book is an essential resource for researchers, scientists, and students interested in enzyme production and their potential applications in industry. Its clear language and detailed analysis of amylase make it an informative read for anyone interested in learning more about the fascinating world of enzymes and their applications.