Temitope Abu

Purification and Characterization of Beta-amylase

From Bacillus Subtilis Isolated from Fermented African Locust Bean

• Broschiertes Buch

Produktbeschreibung

beta-amylase was purified and characterized from Bacillus subtilis isolated from fermented Parkia biglobosa seeds. Purification was achieved using ion exchange DEAE column and gel filtration (Sephadex G-200) chromatography. Effects of temperature; pH and production time on beta-amylase production were investigated, while physicochemical characteristics of the purified enzyme were investigated. The optimum production of beta-amylase was obtained at temperature, pH and time of 37oC, 7.0 and 24 h respectively. The results showed that purified beta-amylase had more enzymatic activity than crude samples from Bacillus subtilis whereby the activity of crude enzyme was 3.21 mM/min/ml while the purified enzyme had an improved activity of 21.46 mM/min/ml. Optimum temperature and pH values of the purified amylase were found to be 50°C and pH 5.0, respectively. pH stability of the enzyme ranged from pH 4.0- 9.0. At pH 5.0 and 7.0 it retained 70% and 60% of its activity respectively after 5 hof incubation. Temperature stability ranged between 40oC and 70oC but most stable at 50oC retaining 64% of its activity after 1 h of incubation. The enzyme exhibited maximum activity on soluble starch and sucrose.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 72
• Erscheinungstermin: 13. August 2015
• Englisch
• Abmessung: 220mm x 150mm x 5mm
• Gewicht: 125g
• ISBN-13: 9783659684258
• ISBN-10: 3659684252
• Artikelnr.: 43737603

Autorenporträt

Abu is a scholar at the Federal University of Technology Akure, Ondo State Nigeria. His academic interest includes Biotechnology, Food Safety and Food security.