This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. The aim of this workshop was to review and discuss the status of our knowledge on the intricate array of enzymes and proteins that allow the replication of the DNA. Since the first discovery of a DNA polymerase in Escherichia coli by Arthur Kornberg twenty eight years ago, a great number of enzymes and other proteins were des cribed that are essential for…mehr
This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. The aim of this workshop was to review and discuss the status of our knowledge on the intricate array of enzymes and proteins that allow the replication of the DNA. Since the first discovery of a DNA polymerase in Escherichia coli by Arthur Kornberg twenty eight years ago, a great number of enzymes and other proteins were des cribed that are essential for this process: different DNA poly merases, DNA primases, DNA dependent ATPases, helicases, DNA liga ses, DNA topoisomerases, exo- and endonucleases, DNA binding pro teins and others. They are required for the initiation of a round of synthesis at each replication origin, for the progress of the growing fork, for the disentanglement of the replication product, or for assuring the fidelity of the replication process. The number, variety and ways in which these proteins inter act with DNA and with each other to the achievement of replication and to the maintenance of the physiological structure of the chromo somes is the subject of the contributions collected in this volume. The presentations and discussions during this workshop reinforced the view that DNA replication in vivo can only be achieved through the cooperation of a high number of enzymes, proteins and other cofactors.
Produktdetails
Produktdetails
Advances in Experimental Medicine and Biology .179
Enzyme Studies of Replication of the Escherichia Coli Chromosome.- Enzymological Studies of the T4 Replication Proteins.- In Vitro Replication of Bacteriophage ?29.- Proteins and Nucleotide Sequences Involved in DNA Replication of Filamentous Bacteriophage.- In Vitro Replication of Rl Miniplasmid DNA.- Analysis of Mu DNA Replicated on Cellophane Discs.- Replication of Bacteriophage Mu and its Mini-Mu Derivatives.- Initiation of DNA Synthesis on Single-Stranded DNA Templates in Vitro Promoted by the Bacteriophage ? O and P Replication Proteins.- Replication In Vitro of Adenovirus DNA.- Characterization of In Vitro DNA Synthesis in an Isolated Chloroplast System of Petunia Hybrida.- In Vitro Synthesis of Cauliflower Mosaic Virus DNA in Viroplasms.- Search of the Enzyme Responsible for the Reverse Transcription Step in Cauliflower Mosaic Virus Replication.- Chromatin Structure and DNA Replication.- Inhibition and Recovery of the Replication of Depurinated Parvovirus DNA in Mouse Fibroblasts.- The Genetics of Adeno-Associated Virus.- Relationship between the Organization of DNA Loop Domains and of Replicons in the Eukaryotic Genome.- Aphidicolin and Eukaryotic DNA Synthesis.- The Origin of DNA Replication of Bacteriophage fl and its Interaction with the Phage Gene II Protein.- Overproduction and Purification of the Gene 2 Product Involved in the Initiation of Phage ?29 Replication.- Autorepression of the dnaA Gene of Escherichia Coli.- Replication Functions Encoded by the Plasmid pSClOl.- How Does Rop Work?.- Gene A Protein of Bacteriophage ?X174 is a Highly Specific Single-Strand Nuclease and Binds Via a Tyrosyl Residue to DNA After Cleavage.- Gene a Protein Interacting with Recombinant Plasmid DNAs Containing 25-30 b.p. of the ?X174 Replication Origin.- How DoesSV40 T Antigen Control Initiation of Viral DNA Replication?.- Studies of the Initiation of DNA Synthesis in Plant and Animal Cells 24.- Function and Properties of RP4 DNA Primase.- De Novo DNA Synthesis by Yeast DNA Polymerase I Associated with Primase Activity.- Paradoxes of In Situ Polyacrylamide Gel Assays for DNA Polymerase Priming.- Association between Primase and DNA Polymerase ? in Murine Cells.- Properties of the Primase Activity of the 9 S DNA Polymerase ? from Calf Thymus.- DNA Polymerase III Holoenzyme of Escherichia Coli: An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases.- Mammalian DNA Polymerase ? Holoenzyme.- DNA Polymerase Activities of Mammalian Cell-Cycle Mutants and "Wild-Type" Cells in Different States of Proliferative Activity and Quiescence.- Correlation of DNA Polymerase Activities with the Initiation of DNA Synthesis.- Synthesis of Catalytically Active Polymerase ? by In Vitro Translation of Calf RNA.- Interaction of DNA Accessory Proteins with DNA Polymerase ? of the Novikoff Hepatoma.- Plasmid Cloning of DNA Polymerase I in Escherichia Coli and Saccharomyces Cerevisiae.- Structural and Functional Properties of DNA Polymerase ? from Calf Thymus.- Functions of DNA Helicases in the DNA Metabolism of Escherichia Coli.- Escherichia Coli DNA Gyrase.- A Unique ATP-Dependent DNA Topoisomerase from Trypanosomatids.- Regenerating Rat Liver Topoisomerase II: Purification of the Enzyme and Catenation of DNA Rings.- Expression of Silent Genes: Possible Interaction between DNA Gyrase and RNA Polymerase.- Poly (ADP-Ribosylation) and DNA Topoisomerase I in Different Cell Lines.- Effect of UV Induced DNA Lesions on the Activity of Escherichia Coli DNA Topoisomerases: A Possible Role of theseEnzymes in DNA Repair.- The Structure of the Bacterial Nucleoid.- Proteins from the Prokaryotic Nucleoid: Biochemical and 1h NMR Studies on three Bacterial Histone-Like Proteins.- The Role of HMG1 Protein in Nucleosome Assembly and in Chromatin Replication.- DNA Binding Proteins in Replicating and Mitotically Arrested Brain Neurons.- Searching for Proteins and Sequences of DNA Replication in Mammalian Cells.- Diadenosine Tetraphosphate and Diadenosine Tetraphosphate-Binding Proteins in Developing Embryos of Artemia.- A Ribonuclease H from Yeast Stimulates DNA Polymerase In Vitro.- Catalytic Activities of Human Poly (ADP-Ribose) Polymerase after SDS-Page.- Fidelity of DNA Replication In Vitro.- Replication of ?X174 DNA by Calf Thymus DNA Polymerase-?: Measurement of Error Rates at the Amber-16 Codon.- Studies on the Role of dam Methylation at the Escherichia Coli Chromosome Replication Origin (oriC).- DNA Methylation and DNA Structure.- Contributors.
Enzyme Studies of Replication of the Escherichia Coli Chromosome.- Enzymological Studies of the T4 Replication Proteins.- In Vitro Replication of Bacteriophage ?29.- Proteins and Nucleotide Sequences Involved in DNA Replication of Filamentous Bacteriophage.- In Vitro Replication of Rl Miniplasmid DNA.- Analysis of Mu DNA Replicated on Cellophane Discs.- Replication of Bacteriophage Mu and its Mini-Mu Derivatives.- Initiation of DNA Synthesis on Single-Stranded DNA Templates in Vitro Promoted by the Bacteriophage ? O and P Replication Proteins.- Replication In Vitro of Adenovirus DNA.- Characterization of In Vitro DNA Synthesis in an Isolated Chloroplast System of Petunia Hybrida.- In Vitro Synthesis of Cauliflower Mosaic Virus DNA in Viroplasms.- Search of the Enzyme Responsible for the Reverse Transcription Step in Cauliflower Mosaic Virus Replication.- Chromatin Structure and DNA Replication.- Inhibition and Recovery of the Replication of Depurinated Parvovirus DNA in Mouse Fibroblasts.- The Genetics of Adeno-Associated Virus.- Relationship between the Organization of DNA Loop Domains and of Replicons in the Eukaryotic Genome.- Aphidicolin and Eukaryotic DNA Synthesis.- The Origin of DNA Replication of Bacteriophage fl and its Interaction with the Phage Gene II Protein.- Overproduction and Purification of the Gene 2 Product Involved in the Initiation of Phage ?29 Replication.- Autorepression of the dnaA Gene of Escherichia Coli.- Replication Functions Encoded by the Plasmid pSClOl.- How Does Rop Work?.- Gene A Protein of Bacteriophage ?X174 is a Highly Specific Single-Strand Nuclease and Binds Via a Tyrosyl Residue to DNA After Cleavage.- Gene a Protein Interacting with Recombinant Plasmid DNAs Containing 25-30 b.p. of the ?X174 Replication Origin.- How DoesSV40 T Antigen Control Initiation of Viral DNA Replication?.- Studies of the Initiation of DNA Synthesis in Plant and Animal Cells 24.- Function and Properties of RP4 DNA Primase.- De Novo DNA Synthesis by Yeast DNA Polymerase I Associated with Primase Activity.- Paradoxes of In Situ Polyacrylamide Gel Assays for DNA Polymerase Priming.- Association between Primase and DNA Polymerase ? in Murine Cells.- Properties of the Primase Activity of the 9 S DNA Polymerase ? from Calf Thymus.- DNA Polymerase III Holoenzyme of Escherichia Coli: An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases.- Mammalian DNA Polymerase ? Holoenzyme.- DNA Polymerase Activities of Mammalian Cell-Cycle Mutants and "Wild-Type" Cells in Different States of Proliferative Activity and Quiescence.- Correlation of DNA Polymerase Activities with the Initiation of DNA Synthesis.- Synthesis of Catalytically Active Polymerase ? by In Vitro Translation of Calf RNA.- Interaction of DNA Accessory Proteins with DNA Polymerase ? of the Novikoff Hepatoma.- Plasmid Cloning of DNA Polymerase I in Escherichia Coli and Saccharomyces Cerevisiae.- Structural and Functional Properties of DNA Polymerase ? from Calf Thymus.- Functions of DNA Helicases in the DNA Metabolism of Escherichia Coli.- Escherichia Coli DNA Gyrase.- A Unique ATP-Dependent DNA Topoisomerase from Trypanosomatids.- Regenerating Rat Liver Topoisomerase II: Purification of the Enzyme and Catenation of DNA Rings.- Expression of Silent Genes: Possible Interaction between DNA Gyrase and RNA Polymerase.- Poly (ADP-Ribosylation) and DNA Topoisomerase I in Different Cell Lines.- Effect of UV Induced DNA Lesions on the Activity of Escherichia Coli DNA Topoisomerases: A Possible Role of theseEnzymes in DNA Repair.- The Structure of the Bacterial Nucleoid.- Proteins from the Prokaryotic Nucleoid: Biochemical and 1h NMR Studies on three Bacterial Histone-Like Proteins.- The Role of HMG1 Protein in Nucleosome Assembly and in Chromatin Replication.- DNA Binding Proteins in Replicating and Mitotically Arrested Brain Neurons.- Searching for Proteins and Sequences of DNA Replication in Mammalian Cells.- Diadenosine Tetraphosphate and Diadenosine Tetraphosphate-Binding Proteins in Developing Embryos of Artemia.- A Ribonuclease H from Yeast Stimulates DNA Polymerase In Vitro.- Catalytic Activities of Human Poly (ADP-Ribose) Polymerase after SDS-Page.- Fidelity of DNA Replication In Vitro.- Replication of ?X174 DNA by Calf Thymus DNA Polymerase-?: Measurement of Error Rates at the Amber-16 Codon.- Studies on the Role of dam Methylation at the Escherichia Coli Chromosome Replication Origin (oriC).- DNA Methylation and DNA Structure.- Contributors.
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