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Buffalo plays an important role in livestock economy over Asia. Among other livestock species the Buffalo is the second most versatile component in relation to existing integrated agricultural farming system in Bangladesh. Reproductive management of buffalo is a foremost concern because of inaccuracy to detect oestrus and pregnancy and all are associated with significant economic losses. Nowadays application of assisted reproductive technologies resembling milk progesterone ELISA, Ultrasonography is successfully improves our understanding on Buffalo Reproduction of Bangladesh. Milk progesterone ELISA was first introduced by Khan et al, (2008) in Bangladesh through Field Fertility Clinic Laboratory (FFC), Bangladesh Agricultural University. Recently milk progesterone ELISA is one of the regularly practiced techniques in Bangladesh to evaluate ovarian cyclicity, oestrus, pregnancy and non-pregnancy status of buffalo, which helps the farmers to maintain the profitable dairy farming.