This in vitro plant culture technique is widely used in numerous practical applications in biotechnology for crop improvement. The impetus for writing this book was to make available an up-to-date text, covering all the theoretical and practical aspects of plant tissue culture for the undergraduate students and researchers of plant sciences and biotechnology. The book explains the basics of tissue culture and the techniques involved in in vitro culturing of plant tissues under controlled environmental conditions. This text book includes ten small chapters profusely illustrated with photos and self-explanatory diagrams. We thank the world recognized Lambert Academic Publishers and those without whose help this book would hardly have been possible. We hope this book would definitely be a useful one for all those who wish to gain knowledge in plant tissue culture. There is no greater wealth than wisdom.