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This workbook has been designed to meet the needs of students for Plant Biotechnology course at various levels of Undergraduate and Graduate studies in different Universities in India and abroad. This workbook covers all the important aspects of Plant Biotechnology from tissue culture techniques to transgenic crop production methods. Workbook comprizes laboratory plan, basic instrumentations and facilities required in tissue culture room, media composition and preparation, sterilization techniques and plant tissue culture techniques like micropropagation, callus cultures, androgenesis, protoplast isolation and suspension culture for secondary metabolite production. To understand applications of Recombinant DNA technology, methods for direct and indirect gene transfer are demonstrated in the workbook. Transgenic crop production protocols for Bt Cotton, Golden Rice, Edible Vaccines and Bioplastic production are illustrated in lucid manner. Principle and theory behind each experiment is explained in easy language. This workbook will definitely help students to acquire basic knowledge of Plant Biotechnology in real time.