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The physicochemical properties and fatty acid composition of Argemone mexicana L. and Celastrus paniculatus Willd. seeds oil were investigated. The oil was extracted and evaluated for different physicochemicals properties and elemental analysis by ICPMS. The fatty acid profile of the oil was determined by GC-MS. The physicochemical analysis of Darudi oil showed less values viz. acid and iodine value as compared to Malkangni oil. The different elemental analysis showed that gold and silver like important elements were found in both the oils at different levels. The Ca, Na, K like elements were also found in both the oils at different levels. The quantitative analysis of two oils showed that fatty acid composition, in which palmitic acid was the most abundant in both the oil followed by linolenic acid in Darudi oil and palmitoleic acid methyl ester was found in Malkangni oil. The qualitative analysis was very similar in their fatty acid composition, in which 26 compounds were foundin Darudi oil, while 26 compounds were found in Malkangni oil. The results suggest that Darudi is suitable as a new source of vegetable oil with potential for domestic and cosmetic industries.