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Our Special Issue reprint highlights the latest advancements in water safety and public health. This curated collection of articles delves into the critical challenges and innovative solutions associated with detecting and managing pathogens in wastewater systems worldwide. Featuring contributions from leading experts in the field, this reprint provides comprehensive insights into the mechanisms of pathogen survival, novel detection methodologies and applications in wastewater-based epidemiology. Perfect for researchers, environmentalists, policymakers and practitioners, this Special Issue offers valuable knowledge to enhance the fight against waterborne diseases and promote sustainable water management practices.