Individual cell and particle analysis in aquatic sciences isinvolved in many aspects of oceanography and limnology,including optical physics of particles, phytoplanktonphysiology and ecology, marine and aquatic microbiology andfood web interactions.This book concentrates on the optimal utilization of flowcytometry and image analysis and the ways in whichoceanographic and limnological problems can be uniquely orbetter addressed using these techniques.
Individual cell and particle analysis in aquatic sciences isinvolved in many aspects of oceanography and limnology,including optical physics of particles, phytoplanktonphysiology and ecology, marine and aquatic microbiology andfood web interactions.This book concentrates on the optimal utilization of flowcytometry and image analysis and the ways in whichoceanographic and limnological problems can be uniquely orbetter addressed using these techniques.
Instrumentation and calibration.- Flow cytometry instrumentation.- Digital microscopy and image analysis: where does it impact on oceanography?.- Applications of microphotometry to optical oceanography.- Calibration and quantitative analysis.- Pigments and fluorescence.- Algal pigment fingerprints: clue to taxon-specific abundance, productivity and degradation of phytoplankton in seas and oceans.- Energy transformation and fluorescence in photosynthesis.- Marine optics and light scattering.- Optics of marine particles and marine optics.- Light scattering properties of cells.- Microbial ecology through individual cell analysis.- Bacterial influence on the variability in the ocean's biogeochemical state: a mechanistic view.- Molecular systematics, microbial ecology and single cell analysis.- From cell to Oceans.- From individual plankton cells to pelagic marine ecosystems and to global biogeochemical cycles.- From the ocean to cells: coccolithophore optics and biogeochemistry.- From cells to the ocean: satellite ocean color.- Individual and bulk analysis of the optical properties of marine particulates: examples of merging these two scales of analysis.- Flow cytometry in oceanography.- Advances in oceanography through flow cytometry.- Conclusion.- Concluding remarks: promises and limitations of individual cell and particle analysis.- List of participants.
Instrumentation and calibration.- Flow cytometry instrumentation.- Digital microscopy and image analysis: where does it impact on oceanography?.- Applications of microphotometry to optical oceanography.- Calibration and quantitative analysis.- Pigments and fluorescence.- Algal pigment fingerprints: clue to taxon-specific abundance, productivity and degradation of phytoplankton in seas and oceans.- Energy transformation and fluorescence in photosynthesis.- Marine optics and light scattering.- Optics of marine particles and marine optics.- Light scattering properties of cells.- Microbial ecology through individual cell analysis.- Bacterial influence on the variability in the ocean's biogeochemical state: a mechanistic view.- Molecular systematics, microbial ecology and single cell analysis.- From cell to Oceans.- From individual plankton cells to pelagic marine ecosystems and to global biogeochemical cycles.- From the ocean to cells: coccolithophore optics and biogeochemistry.- From cells to the ocean: satellite ocean color.- Individual and bulk analysis of the optical properties of marine particulates: examples of merging these two scales of analysis.- Flow cytometry in oceanography.- Advances in oceanography through flow cytometry.- Conclusion.- Concluding remarks: promises and limitations of individual cell and particle analysis.- List of participants.
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