Non-Viral Gene Delivery Vectors

Methods and Protocols
Herausgegeben:Candiani, Gabriele

• Broschiertes Buch

Produktbeschreibung

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery-also termed reversetransfection-and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.

Produktdetails

• Methods in Molecular Biology 1445
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8118-2
• Softcover reprint of the original 1st ed. 2016
• Seitenzahl: 304
• Erscheinungstermin: 30. Mai 2018
• Englisch
• Abmessung: 254mm x 178mm x 17mm
• Gewicht: 576g
• ISBN-13: 9781493981182
• ISBN-10: 1493981188
• Artikelnr.: 52906146

Inhaltsangabe

Physical Chemical and BiomolecularMethods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitrofor the Gene Therapy Applications.- Cationic Lipid-Based Nucleic Acid Vectors.- Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPELiposomes and Gemini Micelles.- Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA.- Elaboration and Physicochemical Characterization of Niosome-BasedNioplexes for Gene Delivery Purposes.- Quantitative Intracellular Localization of Cationic Lipid-NucleicAcid Nanoparticles with Fluorescence Microscopy.- TargetedDelivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.- Lipoplexes Strengthened by AnionicPolymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipidsand Anionic Polymers.- Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.- PolyallylamineDerivatives: Novel Non-Toxic Transfection Agents.- BiodegradableThree-Layered Micelles and Injectable Hydrogels.- Cationic Lipid-CoatedPolyplexes ("Lipopolyplexes") for DNA and Small RNA Delivery.- Preparation of Targeted AnionicLipid-Coated Polyplexes for MicroRNA Delivery.- Characterizationand Investigation of Redox-Sensitive Liposomes for Gene Delivery.- From ArtificialAmino Acids to Sequence-Defined Targeted Oligoaminoamides.- Gene DeliveryMethod Using Photo-Responsive Poly(beta-Amino Ester) as Vectors.- Thermo-ResponsivePolyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for SystemicGene Therapy.- Application of Polyethylenimine-Grafted Silicon NanowireArrays for Gene Transfection.