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This Special Issue contributes to filling knowledge gaps regarding NUS in horticultural and ornamental systems, as well as in landscapes, by collecting original research papers dealing with the relevance of NUS to the following topics: biodiversity and conservation; genetics and breeding; characterization, propagation, and ecophysiology; cultivation techniques and systems; landscape protection and restoration; product and process innovations; biochemistry and composition; and postharvest factors affecting their end-use quality.