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A complete overview of the latest developments in optical microscopy that enable structure determination at the nanoscale, this timely book draws contributions from top experts in their respective fields, including those who have either led the current upsurge in research or who have laid the foundation for the development of modern microscopes and techniques. Contributors offer state-of-the-art treatment of critical topics in nanoscopy, from imaging and manipulation to spectroscopy and visualization. Interdisciplinary in scope and approach, the book combines the science and tools of biologists, physicists, and engineers.