Microtechnologies to fate-map single stem cells and their progeny
Stefan A. Kobel
Broschiertes Buch

Microtechnologies to fate-map single stem cells and their progeny

How to culture, track and individually analyze single stem cells and their progeny with micro-devices

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Stem cells have the unique ability to produce more of themselves (i.e. to self-renew ) and to generate specialized tissue cells. In order to use this dual ability for regenerative medicine or in vitro tissue models, we have to understand the mechanisms that regulate stem cell behavior at a single cell level. However, current in vitro methodologies to study single cells are either limited by their throughput or cannot efficiently track single stem cells over long time periods and then still distinguish their fate. This thesis addressed these shortcomings by developing miniaturized devices to ha...