MicroRNAs as Tools in Biopharmaceutical Production

Herausgegeben:Barron, Niall

• Gebundenes Buch

Produktbeschreibung

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Produktdetails

• Verlag: Springer / Springer Netherlands
• Artikelnr. des Verlages: 86131300, 978-94-007-5127-9
• 2012
• Seitenzahl: 136
• Erscheinungstermin: 16. November 2012
• Englisch
• Abmessung: 241mm x 160mm x 13mm
• Gewicht: 381g
• ISBN-13: 9789400751279
• ISBN-10: 9400751273
• Artikelnr.: 35996225

Inhaltsangabe

Preface.-1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines.-2 Biogenesis of Mammalian miRNA.-3 Target prediction algorithms and bioinformatics resources for miRNA studies.-4 The CHO miRNA transcriptome.-5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes.-6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies.-7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production.-8 Manipulating Levels of Specific microRNAs in Mammalian Cells.- Index.