Metastasis Research Protocols

Herausgegeben:Brooks, Susan A.; Schumacher, Udo

• Broschiertes Buch

Produktbeschreibung

In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systemsin turn must of course be put into perspective relative to their roles in entire living organisms.

Produktdetails

• Methods in Molecular Medicine 58
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-099-1
• Softcover reprint of hardcover 1st ed. 2001
• Seitenzahl: 310
• Erscheinungstermin: 9. November 2010
• Englisch
• Abmessung: 229mm x 152mm x 19mm
• Gewicht: 483g
• ISBN-13: 9781617370991
• ISBN-10: 1617370991
• Artikelnr.: 32201257

Inhaltsangabe

Selection/Segregation Cell Populations for in Vitro and in Vivo Assays of Metastatic Behavior.- Cell Separations by Flow Cytometry.- Immunomagnetic Cell Separation.- Genetic Modification of Cell Lines to Enhance Their Metastatic Capability.- In Vitro Assays of Metastatic Behavior.- Cell Aggregation Assays.- Cell Migration and the Boyden Chamber.- Quantification of Cell Motility.- In Vitro Invasion Assay Using Matrigel®.- Membrane Invasion Culture System.- Collagen Invasion Assay.- Chick Heart Invasion Assay.- Adhesion of Tumor Cells to Matrices and Endothelium.- Assessment of Angiogenic Factors The Chick Chorioallantoic Membrane Assay.- Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis.- Galectin-3 Binding and Metastasis.- Animal Models of Metastasis.- Basic Principles for the Study of Metastasis Using Animal Models.- The Chick Embryo Metastasis Model.- GeneTransfection for Metastasis Research Using Animal Models.- Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice.- Xenograft Models in Immunodeficient Animals.- Xenograft Models in Immunodeficient Animals.- Syngeneic Murine Metastasis Models.- Transgenic Animal Models.- Clinically Accurate Orthotopic Mouse Models of Cancer.- Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo.- Green Fluorescent Protein for Metastasis Research.

Rezensionen

This book is a valuable addition to the literature as it brings together key people in the field of cancer metastasis to provide practical step-by-step protocols. . . This book is written in such as way that it would be useful to beginning students and to anyone curious about the methods that are available to study the key steps in cancer metastasis. This book would also be useful to seasoned researchers as it brings together a number of different methodologies in one easy-to-read book. . . This is a very practical book covering areas of research in cancer invasion and metastasis. The greatest strength of the book is in collecting the different protocols that are useful in studying cellular behavior in one easy-to-read book.."-Weighted Numerical Score: 91 - 4 Stars!-Doody's Health Sciences Book Review Journal

"These volumes should be of interest for all medical and academic libraries where cancer research is performed." - E-Streams