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Fueled by renewable feedstocks and powered from wind, sun, water and biomass the bioeconomy produces biobased chemicals and thus has the potential to create a sustainable chemical industry. In this dissertation, we investigated pseudomonads, a promising microbial platform organism for the bioeconomy. The complete genome of P. putida S12 was published and building blocks, namely styrene and anthranilate, which serve as precursors for bulk and high value products, were produced with pseudomonads.