Carola Hunte / Gebhard von Jagow / Hermann Schagger (eds.)

Membrane Protein Purification and Crystallization

A Practical Guide
Herausgeber: Hunte, Carola; Schagger, Hermann; Jagow, Gebhard von

• Broschiertes Buch

Produktbeschreibung

- Provides general guidelines and strategies for isolation and crystallization of membrane proteins - Gives detailed protocols that have wide application, and low specialized equipment needs - Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitopetagged proteins

Produktdetails

• Verlag: Wiley
• 2nd edition
• Seitenzahl: 316
• Erscheinungstermin: 19. Dezember 2002
• Englisch
• Abmessung: 239mm x 205mm x 15mm
• Gewicht: 599g
• ISBN-13: 9780123617767
• ISBN-10: 0123617766
• Artikelnr.: 21262807

Inhaltsangabe

I. Strategies and Techniques Purification Strategies for Membrane Proteins
Chromatographic Techniques and Basic Operations in Membrane Protein
Purification Production and purification of recombinant membrane proteins
SDS Electrophoresis Techniques Blue-Native Electrophoresis Preparative
Isoelectric Focussing Membrane protein crystallization
II. Discussion of Selected Isolation Protocols Lipid Dependent Inactivation
and Reactivation of Bovine complex III Purification of affinity-epitope
tagged G-protein coupled receptors Purification of NhaA, the Na+/H+
Antiporter of Escherichia coli for 3D or 2D Crystallization Purification of
the yeast cytochrome bc1 complex
III. Crystallization of Membrane Proteins Antibody fragment-mediated
crystallization of membrane proteins Crystallization of Wolinella
succinogenes quinol:fumarate reductase Ba3-type cytochrome-c oxidase from
Thermus thermophilus purification, crystallization, and crystal
transformation Two-dimensional crystallization of membrane proteins: a
practical guide In cubo crystallization of membrane proteins