Mass Spectrometry in the Biological Sciences covers the most recent technological and applied developments in the area, including both ionization techniques and ion analysis. It introduces and reviews some of the newer ionization methods, describes the major instrumentation involved in mass analysis, and presents the scope of the technology in biology, medicine, and environmental science. Specific examples are given for a number of topics. It also deals with recent achievements in the on-line combination of separation techniques such as gas chromatography, liquid chromatography, and supercritical fluid technology.…mehr
Mass Spectrometry in the Biological Sciences covers the most recent technological and applied developments in the area, including both ionization techniques and ion analysis. It introduces and reviews some of the newer ionization methods, describes the major instrumentation involved in mass analysis, and presents the scope of the technology in biology, medicine, and environmental science. Specific examples are given for a number of topics. It also deals with recent achievements in the on-line combination of separation techniques such as gas chromatography, liquid chromatography, and supercritical fluid technology.
I: Methods in Mass Spectrometry.- 1. Ion Formation by Fast Atom Bombardment Mass Spectrometry.- 2. Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry of Biological Molecules.- 3. Matrix-Assisted Laser Desorption Mass Spectrometry on a Magnetic Sector Instrument Fitted with an Array Detector.- 4. Electrospray Ionization Mass Spectrometry: Protein Structure.- 5. Applications of Quantum Chemistry in Mass Spectrometry.- 6. Proton Affinities and Intrinsic Basicities of Alanine and Glycine Studied by Means of AM1 and PM3 Methods.- II: Instrumentation for Mass Analysis and Detection.- 7. Energy-Isochronous Time-of-Flight Mass Spectrometers.- 8. The Detection of High Mass-to-Charge Biological Ions by Fourier Transform Mass Spectrometry: Issues and Routes for Instrument Improvement.- 9. Quadrupole Mass Filters, Quadrupole Ion Traps and Fourier Transform Ion Cyclotron Resonance Spectrometers.- 10. Energy Shifts in Collisional Activation.- 11. Hybrid Instruments of Unusual Geometry.- III: Application to Biomolecules.- 12. Continuous-Flow Fast Atom Bombardment Mass Spectrometry: Applications to Dynamic Systems.- 13. Hybrid Tandem Mass Spectrometry of Peptides.- 14. Mass Spectrometry in the Study of Advanced Glycation End Products.- 14. Nucleic Acids: Overview and Analytical Strategies.- 15. Monitoring of Human Exposure to Xenobiotics; Identification and Quantification of Cancer Initiators in Vivo.- 16. The Role of Mass Spectrometry in Biomonitoring Exposure to Carcinogens.- 17. Characterization of Ceramide Mixtures by Fast Atom Bombardment and Tandem Mass Spectrometry.- 18. Bioorganic Studies of a New Photoreceptor Structure.- IV: Coupling of Chromatography to Mass Spectrometry.- 19. Gas Chromatography and Mass Spectrometry Coupling.- 20. Recent Developments in Combined Supercritical Fluid Chromatography/Mass Spectrometry (SFC/MS).- 21. GC-MS Analysis of Volatile Compounds of Mesir.- 22. The Application of Mass Spectrometry to Food and Nutrition Research.
I: Methods in Mass Spectrometry.- 1. Ion Formation by Fast Atom Bombardment Mass Spectrometry.- 2. Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry of Biological Molecules.- 3. Matrix-Assisted Laser Desorption Mass Spectrometry on a Magnetic Sector Instrument Fitted with an Array Detector.- 4. Electrospray Ionization Mass Spectrometry: Protein Structure.- 5. Applications of Quantum Chemistry in Mass Spectrometry.- 6. Proton Affinities and Intrinsic Basicities of Alanine and Glycine Studied by Means of AM1 and PM3 Methods.- II: Instrumentation for Mass Analysis and Detection.- 7. Energy-Isochronous Time-of-Flight Mass Spectrometers.- 8. The Detection of High Mass-to-Charge Biological Ions by Fourier Transform Mass Spectrometry: Issues and Routes for Instrument Improvement.- 9. Quadrupole Mass Filters, Quadrupole Ion Traps and Fourier Transform Ion Cyclotron Resonance Spectrometers.- 10. Energy Shifts in Collisional Activation.- 11. Hybrid Instruments of Unusual Geometry.- III: Application to Biomolecules.- 12. Continuous-Flow Fast Atom Bombardment Mass Spectrometry: Applications to Dynamic Systems.- 13. Hybrid Tandem Mass Spectrometry of Peptides.- 14. Mass Spectrometry in the Study of Advanced Glycation End Products.- 14. Nucleic Acids: Overview and Analytical Strategies.- 15. Monitoring of Human Exposure to Xenobiotics; Identification and Quantification of Cancer Initiators in Vivo.- 16. The Role of Mass Spectrometry in Biomonitoring Exposure to Carcinogens.- 17. Characterization of Ceramide Mixtures by Fast Atom Bombardment and Tandem Mass Spectrometry.- 18. Bioorganic Studies of a New Photoreceptor Structure.- IV: Coupling of Chromatography to Mass Spectrometry.- 19. Gas Chromatography and Mass Spectrometry Coupling.- 20. Recent Developments in Combined Supercritical Fluid Chromatography/Mass Spectrometry (SFC/MS).- 21. GC-MS Analysis of Volatile Compounds of Mesir.- 22. The Application of Mass Spectrometry to Food and Nutrition Research.
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