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Enzymes are biological catalysts that lower the activation energy of biological reactions.Bacteria can be used in the industrial production of several enzymes.Through my work, I would like to give a short description of Isolation of Lipase enzyme producing bacteria from soil, Production of enzyme, Characterization of enzyme and its Industrial Application.Substrate used in enzyme production must be economical.I have also made an attempt to try various substrates which is cheaper than the one used in the actual production.