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Laccases are most stable and powerful biocatalysts with broad applications. It is a copper containing, organic solvents resistance, oxidoreductase enzyme, having potential ability to oxidize the phenolic and non-phenolic compounds. Laccases play an important role in food industry, paper and pulp industry, textile industry, synthetic chemistry, cosmetics, soil bioremediation and biodegradation of environmental phenolic pollutant. Soil samples were collected from different places and The fungi which is selected for study is confirmed by fungal stain is to be Fusarium sp. P 15 were employed for further production of Laccase studies.