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Antibiotics resistant have been a global threat in healthcare. These days, bacterial pathogens causing common clinical infections have acquired resistance against almost all currently available antibiotics. New efficient therapeutic approaches are mandatory; otherwise the pre-antibiotic era like situation is imminent. Use of lytic bacteriophages and their products as a therapeutic or biocontrol agents have been renewed of interest and considered as an alternatives to treat the multidrug resistant (MDR) bacterial pathogens. Some phage products have already got regulatory approval particularly to be used in food safety; many others are on the stage of preclinical and clinical trials. This book highlighted the details of methodology related to the isolation, characterization and application of lytic bacteriophages as an effective biocontrol agent.