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The main objectives of this book are to proved annotation of dengue virus serotypes genomes at nucleotide level for better understanding and ease organization. Genome Organization and Genome Mapping of all serotypes of Dengue Virus to sort out comparative genome analysis. Distribution of structural and non-structural proteins on the basis of Size. DENV Intra-specie Single Nucleotide Polymorphism in C/PrM region of Pakistan sequences, in aim to compare SNP with reference DENV genome. Protein Level Annotation to sort out motifs of super families and domains aligned between four serotypes of Dengue virus. Functional Annotation of proteins to find out functions of domains and motifs. Evolutionary Analysis of DENV C/PrM region (in focus to Pakistan C/PrM sequences) and DENV four serotypes whole genomes of whole world. Process Level Annotation. To check the importance of N-Glycosylation and O-Glycosylation in the dengue virus disease virulence.