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The aim of this work is to standardise the plant Rumex dentatus by performing the phyto-chemical investigations & estimation of sugar, starch, tannins, phenolics, flavonoids & performing fluorescence analysis, ash & extractive value for powdered leaves of Rumex dentatus. The fluorescence analysis of drug shows various colors in different reagents in day light, 254nm & 365nm, the ash value was found to be 20.45%, Water soluble ash value and Acid insoluble ash value were found to be 9.7% & 5.2% respectively the extractive value in hexane, alcohol, & water soluble extract was found to be 6.75%, 9.58%, 15.55%. The sugar and starch content in powdered leaves of Rumex dentatus was found to be 0.037% & 5.78%. The UV spectroscopic estimation of tannins, phenolics, flavonoids content in powdered leaves of Rumex dentatus was found to be 1.15%, 56.008%, 0.353%. TLC and HPTLC were performed for quantative estimation of emodin in different extracts.