In-Cell NMR Spectroscopy

From Molecular Sciences to Cell Biology
Herausgeber: Ito, Yutaka; Shirakawa, Masahiro; Dötsch, Volker

• Gebundenes Buch

Produktbeschreibung

In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives. Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.

Produktdetails

• Verlag: RSC Publishing
• Seitenzahl: 305
• Erscheinungstermin: 13. Dezember 2019
• Englisch
• Abmessung: 236mm x 163mm x 25mm
• Gewicht: 635g
• ISBN-13: 9781788012171
• ISBN-10: 1788012178
• Artikelnr.: 54845374

Inhaltsangabe

In-cell NMR Spectroscopy: From 2001 to Now
Protein NMR in Escherichia coli
In-cell NMR in Eukaryotic Cells - I (Introduction from Outside of Cells)
In-cell NMR in Eukaryotic Cells - II (Direct Protein Expression)
Protein Structure Determination in Living Cells from NOE-derived Distance Restraints
In-cell Structure Determination Using Paramagnetic NMR Spectroscopy
Bioreactor System for In-cell NMR Observation
19F In-cell NMR
Cellular Solid-state NMR Spectroscopy
In-cell EPR
In-cell NMR: Spectral Optimization and Population Adjustment by Rational Protein Engineering
Protein Stability and Weak Intracellular Interactions
Protein Folding, Maturation and Redox State
All-atom Molecular Dynamics Simulation of Proteins in Crowded Environments
Primary Drug Screening by In-cell NMR Spectroscopy
In-cell NMR Spectroscopy of Nucleic Acids