Saima Feroz

Improving the Thermostability of Baeyer Villiger Monooxygenases

• Broschiertes Buch

Produktbeschreibung

CHMO originating from Acinetobacter NCIB 9871 was chosen for this study. Mutations to stabilize helical structure motifs in particular were predicted by sequence comparison with the structure of PAMO from Thermobifida. Certain combinatorial and rational protein design approaches were applied for the development of several generations of mutants. Parallel screening methods, both on whole-cells and crude cell extracts, were utilized to assess biocatalytic performance. These mutants were screened for their thermostability according to the protocols established for this study. ThermoFAD experiments indicated the presence of two native active stages, N1 and N2 of the enzyme, which was followed by unfolded stage (U) and then deactivated (D) form, if denaturing condition stayed. Shelf life of the mutant enzymes at 30°C was improved from 80 hours (WT) to 722 hours (mutant). An increase of more than 1400 % in the yield during the life time of enzyme, over the wild type was achieved. The generality of the consensus based approach for the improvement of thermal stability of biocatalysts was successfully applied to a flavin monooxygenase for the first time.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 276
• Erscheinungstermin: 29. Juni 2013
• Englisch
• Abmessung: 220mm x 150mm x 17mm
• Gewicht: 429g
• ISBN-13: 9783659416989
• ISBN-10: 3659416983
• Artikelnr.: 39894863

Autorenporträt

Dr. Saima FEROZ is a promising molecular biologist and Assistant Professor at CIIT Islamabad Pakistan. She acquired her PhD from Technical University of Vienna,Austria in 2013. She was awarded a PhD fellowship (HEC Pakistan),the EMBO research award 2010 and was among the 15 winners of IYC 2011 Sonderstipendium der GÖCH by Austrian Chemical Society.