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The main objective of reproductive biotechnology for farm animals is increasing reproductive efficiency and rates of animal genetic improvement. Arresting of development for oocytes development stages is one of the important problem facing researchers and reduce the rate of maturation and fertilization in vivo and in vitro. Many researchers are moving towards adding different supplementations in medium to raise maturation and fertilization rate in vitro. Since ten years ago, many researchers investigated the role of Leptin in reproduction and fertility. Leptin is a cytokine hormone, 167 amino acids and 16 K-Da. It is secreted primarily by adipose tissues. Leptin increases the proportion of oocytes that is developed to blastocysts. Adding Leptin to IVM medium by different levels enhances the maturation rate of in vitro rabbit oocytes. In this work, author was trying to investigate the effect of Leptin supplementation at different levels in IVM medium on rabbit oocyte maturation.