High Throughput Screening

Methods and Protocols
Herausgegeben:Janzen, William P.

• Broschiertes Buch

Produktbeschreibung

This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening, high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chaptersinclude introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.

Produktdetails

• Methods in Molecular Biology 1439
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8113-7
• 3. Aufl.
• Seitenzahl: 320
• Erscheinungstermin: 31. Mai 2018
• Englisch
• Abmessung: 254mm x 178mm x 18mm
• Gewicht: 610g
• ISBN-13: 9781493981137
• ISBN-10: 1493981137
• Artikelnr.: 52904404

Inhaltsangabe

Design and Implementation of High Throughput Screening Assays.- Characterization of InhibitorBinding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method.- High Throughput Screening UsingMass Spectrometry within Drug Discovery.- Structure-Based Virtual Screeningof Commercially Available Compound Libraries.- AlphaScreen-Based Assays:Ultra-High Throughput Screening for Small Molecule Inhibitors of ChallengingEnzymes and Protein-Protein Interactions.- Instrument Quality Control.- Application of FluorescencePolarization in HTS Assays.- Time Resolved Fluorescence Assays.- Protein Kinase SelectivityProfiling Using Microfluid Mobility Shift Assays.- Screening for Inhibitors ofKinase Autophosphorylation.- A Fluorescence-Based HighThroughput Screening Assay to Identify Growth Inhibitors of the PathogenicFungus Aspergillus fumigatus.- Mycobacterium tuberculosisHigh Throughput Screening.- Identification ofState-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR BasedAssay.- A Luciferase Reporter GeneSystem for High Throughput Screening of Gamma-Globin GeneActivators.- A High Throughput Flow CytometryAssay for Identification of Inhibitors of 3', 5'-Cyclic Adenosine MonophosphateEfflux.- High Throughput Cell ToxicityAssays.- BRETⁿ: NanoLuc-BasedBioluminescence Resonance Energy Transfer Platform to Monitor Protein-ProteinInteractions in Live Cells.- Application of Imaging-BasedAssays in Microplate Formats for High Content Screening.