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"Heavy Metals and Physico-chemical Assessment of Water, Sediments, and Oreochromis Niloticus (Tilapia) in Maragondon River, Philippines" Maragondon River serves as source of water supply for domestic, agricultural, and industrial purposes which also provides livelihood among locals. However, anthropogenic activities might affect the quality of water. Physico-chemical assessment revealed that characteristics of water are all within the permissible limit set by DENR standards for Class B water. Both copper (Cu) and lead (Pb) showed no significant difference and within the Klokes Maximum Allowable Limit at 20-100 ppm. The heavy metal concentrations in the tissues of Orechromis niloticus (Tilapia) were able to pass the recommended limits joint by WHO/FAO, Food Standards Programme Codex Committee on contaminants in food.