Flurina Christina Clement, Ramiro Dip, Hanspeter Naegeli

Glycidamide-induced expression signature

Transcriptional fingerprint of glycidamide, the reactive metabolite of the widespread food carcinogen acrylamide

• Broschiertes Buch

Produktbeschreibung

Recent findings of acrylamide in foods have sparked new interest in assessing human health hazards due to long-term exposure to this vinyl compound. Acrylamide is tumorigenic at high doses in rodents but cancer risk projections in the human population remain uncertain as the molecular pathogenesis of acrylamide at the low dietary uptake is not understood. The present work addresses the question whether transcriptional responses amplify the known genotoxicity of acrylamide. Using DNA microarrays and PCR validations, we assessed genome-wide messenger profiles induced in human cells by acrylamide and glycidamide, its more reactive metabolite. The expression changes are characterized by the up-regulation of cytoprotective factors including the GSH system and multiple antioxidants. Low-dose experiments indicate that EPHX1 represents the most sensitive biomarker for glycidamide exposure. At higher concentrations, glycidamide induces markers of tumor progression, while growth suppressors and cell adhesion molecules are down-regulated. These findings indicate that tumor-promoting transcriptional signatures may be expected only at higher doses that exceed the ordinary dietary exposure.

Produktdetails

• Verlag: Südwestdeutscher Verlag für Hochschulschriften
• Seitenzahl: 56
• Erscheinungstermin: 12. August 2015
• Deutsch
• Abmessung: 220mm x 150mm x 4mm
• Gewicht: 91g
• ISBN-13: 9783838106304
• ISBN-10: 383810630X
• Artikelnr.: 26459112

Autorenporträt

Dr. med. vet. Flurina C. Clement, PhD Student;Dr. med. vet. Ramiro Dip, PhD, Principle Investigator;Prof. Dr. med. vet. Hanspeter Naegeli, Head of Toxicology Division;Institute of Veterinary Pharmacology and Toxicology, Universityof Zurich