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Genetic engineering is the alteration of genome using biotechnology. It is a compilation of technologies used to modify the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. This book elucidates new techniques and their applications in a multidisciplinary approach keeping the focus on genetic engineering. Also included in this book is a detailed explanation of the various concepts and applications of this discipline in different fields like medicine, manufacturing, gene therapy, etc. The various studies that are constantly contributing towards advancing technologies and evolution of this field are examined in detail. Researchers and students in this field will be assisted by this book.