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Master's Thesis from the year 2018 in the subject Biology - Micro- and Molecular Biology, grade: 2, , course: Feed Biotechnology- Animal nutrition, Master of Animal Science and Technology, language: English, abstract: Not all Bacillus strains are equally resistant to the harsh evironment in the gastrointestial tract besides there are also variations in enzyme activity, and antimicrobial activity, hence selection of appropriate Bacillus strains is essential for the effectiveness of probiotic additives for use in animal feed. The purpose of this study was to describe in vitro probiotic properties such as acid tolerance, bile tolerance, antimicrobial activity and safety, of two Bacillus strains, B. glycinifermentans SK4275 and B. paralicheniformis SK4278 isolated from elephant grass in addition to their cellulolytic enzyme activity. The effect of live B. glycinifermentans SK 4275 on in vitro fermentation of elephant grass (P ennisetum purpureum) was also investigated.