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The study was designed to investigate the antimicrobial and anti-inflammatory efficiency of aqueous extract of dried papaya and castor seeds, which are known to possess various therapeutic properties and economical applications. Microtiter Plate Assay method was used to find out antimicrobial analysis. The aqueous extract has a good antibacterial activity against Staphylococcus aureus and the inhibitory percentage for papaya and castor are 79.9% and 85% respectively with the maximum concentration used in this. The anti-inflammatory action is done by HRBC Membrane Stabilization Assay and papaya and castor seed extract showed 56% and 46% inhibition with the maximum concentration used in this. Thus the seeds can be suggested us the preventive agents in diseases and conditions like cancer, edema, tumour, diabetics, anti-viral, cardiovascular diseases, neuro- degenerative diseases to certain extent.